A culture workflow can be supplied with HEPES, DMSO or glycerol, glutamine and sodium pyruvate, trypsin USP 1:250 and selection antibiotics. Basal, serum-free and specialty media are on the cell culture media page.
Items that can be lined up
- Buffers: HEPES and DPBS. See how to choose a buffer.
- Freezing: DMSO and cell-culture glycerol.
- Supplements: L-glutamine, sodium pyruvate and amino acids.
- Detachment: trypsin USP 1:250.
- Selection: G418, hygromycin, penicillin salts and streptomycin. See antibiotics.
Basal and serum-free media, balanced salt solutions and antibiotic solutions: see cell culture media.
Questions on this application
Which HEPES concentration is typical?
10–25 mM in most mammalian culture media.
Which DMSO grade for freezing cells?
Ask for sterile cell-culture grade, usually used at 5–10%.
For research or industrial use only. Not for human or animal diagnosis or treatment, and not for drug or food use. Grade, pack size and lead time follow the supplier batch COA / SDS. Figures on this site are general industry references, not a factory specification.