G418 sulfate

CAS

108321-42-2

Form

Sulfate raw material

Potency

On the supplier COA, which Catch-Chem forwards and does not assay

What it is

G418 (Geneticin) is an aminoglycoside related to gentamicin. It binds the 80S ribosome and blocks elongation, killing eukaryotic cells. The neo gene (aminoglycoside 3'-phosphotransferase, APH(3')-II) inactivates it by phosphorylation, so only transfected cells survive.

Where it is used

• Stable mammalian cell lines: typically 200–800 µg/mL active G418 for selection, often about 400 µg/mL, over roughly 1–3 weeks.
• Maintenance of established lines at a reduced dose, commonly about half the selection level.
• Yeast selection with kanMX cassettes, often around 200 µg/mL.
• Plant and other eukaryotic systems carrying nptII. Set-up is covered in how selection is set up.

Handling tips

• Calculate the dose on active G418 (µg active per mg on the COA), not on powder weight.
• Run a kill curve for each cell line and each new lot.
• Prepare a stock around 50 mg/mL active in water or buffer, filter-sterilize, and store at 2–8 °C or −20 °C.

What to specify when ordering

• Potency (µg/mg) and cell-culture grade.
• Powder or sterile solution with concentration.
• Pack size.

Properties and storage

Formula

C20H40N4O10·2H2SO4

Molecular weight

692.70

Appearance

White to off-white powder

Solubility

Freely soluble in water (stocks usually 50–100 mg/mL)

Storage

Powder 2–8 °C, dry; filter-sterilised solution 2–8 °C

Typical packs

1 g, 5 g, 25 g

Typical literature values. The batch COA / SDS governs.

Before you inquire

Pack sizes and catalogue numbers

Prices on request; lead time confirmed on the quotation.

For research or industrial use only. Not for human or animal diagnosis or treatment, and not for drug or food use. Grade, pack size and lead time follow the supplier batch COA / SDS. Figures on this site are general industry references, not a factory specification.