- CAS
- 9002-07-7
- Listed grade
- USP, 1:250
- Not the same as
- A 1:4000 mixed digestive enzyme
This is the USP 1:250 trypsin used to passage cells and, in proteomics, to digest proteins. It is a different product from a 1:4000 mixed digestive enzyme. For cell culture, consider whether EDTA is used and how the enzyme is inactivated.
What it is
Trypsin is a pancreatic serine protease that cleaves peptide bonds on the carboxyl side of lysine and arginine, except before proline. The USP 1:250 designation describes a porcine pancreatic preparation by its digestive strength; it also carries other pancreatic proteases, which helps in dissociating cells.
Where it is used
- Detaching adherent cells for passaging, typically 0.25% or 0.05% trypsin, often with EDTA (about 0.02%, 0.5 mM), for a few minutes at 37 °C.
- Dissociating primary tissue into single cells, sometimes with collagenase.
- Bulk protein digestion. LC-MS peptide mapping usually calls for TPCK-treated or sequencing-grade trypsin, which is a different specification.
Handling tips
- Rinse cells with calcium- and magnesium-free PBS or DPBS first; divalent ions and serum inhibit detachment.
- Stop digestion with serum-containing medium or soybean trypsin inhibitor; over-exposure damages surface proteins.
- Filter-sterilize solutions (do not autoclave), aliquot and store at −20 °C; avoid repeated thawing.
What to specify when ordering
- Grade: USP 1:250 or a higher-purity grade, and source species.
- Powder or sterile solution, with or without EDTA and phenol red.
- Pack size. Context: cell culture.
Properties and storage
- Formula
- —
- Molecular weight
- about 23.3 kDa
- Appearance
- White to pale yellow powder
- Solubility
- Soluble in water and balanced salt solutions
- Storage
- 2–8 °C, dry; solutions aliquoted at −20 °C
- Typical packs
- 25 g, 100 g, 500 g
Typical literature values. The batch COA / SDS governs.
Before you inquire
Is this 1:4000?
No. The listed item is USP 1:250.
0.25% or 0.05% trypsin?
0.25% works faster for strongly adherent lines. 0.05% is gentler and common for sensitive or routinely passaged cells. Titrate time against detachment.
Is this suitable for mass-spectrometry digestion?
Crude 1:250 trypsin contains chymotrypsin and other proteases. For LC-MS, ask for TPCK-treated or sequencing-grade trypsin.
Pack sizes and catalogue numbers
CTH10033-1KG
Prices on request; lead time confirmed on the quotation.
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Send an inquiryFor research or industrial use only. Not for human or animal diagnosis or treatment, and not for drug or food use. Grade, pack size and lead time follow the supplier batch COA / SDS. Figures on this site are general industry references, not a factory specification.