A routine SDS-PAGE inquiry starts with ultra-pure SDS and Tris. Small peptides can ask for Tricine. A cold system can ask for LDS. Staining uses Coomassie R-250 or G-250. Transfer checking uses Ponceau S. Precast gels and markers: see proteomics.
Where each item sits
- Ultra-pure SDS and LDS
- Tris / Tris-HCl. Glycine is under media supplements; culture grade and electrophoresis grade are different inquiries.
- Tricine is on the buffer page. Urea is under lysis.
- Coomassie and Ponceau S are under stains.
Reducing samples can inquire DTT. Antibodies: see proteomics.
Questions on this application
Do you have precast gels?
Yes, see the proteomics page. State percentage, buffer system and well count.
G-250 or R-250?
Bradford and colloidal stains usually ask for G-250. Routine SDS-PAGE band staining usually asks for R-250.
For research or industrial use only. Not for human or animal diagnosis or treatment, and not for drug or food use. Grade, pack size and lead time follow the supplier batch COA / SDS. Figures on this site are general industry references, not a factory specification.