DTT

CAS

27565-41-9

Role

Disulfide reduction and RNase inhibition

Handling

Solutions are frozen and not freeze-thawed repeatedly

What it is

DTT (dithiothreitol, Cleland's reagent) is a dithiol reducing agent. After reducing a disulfide it closes into a stable six-membered ring, which drives the reaction to completion and makes it far more effective than 2-mercaptoethanol at equal concentration. It works best above pH 7, since the reactive species is the thiolate.

Where it is used

• Protein buffers and purification, typically 1–10 mM to keep cysteines reduced.
• SDS-PAGE sample buffer, commonly 50–100 mM to break disulfides before loading.
• Proteomics: reduction at about 5–10 mM before alkylation with iodoacetamide.
• Enzyme reaction buffers, including reverse transcription, and nucleic-acid lysis mixes with guanidine isothiocyanate.

Handling tips

• DTT oxidizes in air. Make a 1 M stock in water, aliquot and store at −20 °C; avoid repeated freeze-thaw.
• Keep DTT low (about 1 mM or less) with Ni-NTA resins, which it can reduce.
• Store the powder cold, dry and sealed; it has a noticeable sulfur odor.

What to specify when ordering

• Purity and grade (general or molecular biology).
• Solid or ready solution with concentration.
• Pack size.

Properties and storage

Formula

C4H10O2S2

Molecular weight

154.25

Appearance

White crystalline powder with a faint thiol odour

Solubility

Freely soluble in water and ethanol

Storage

2–8 °C, dry, sealed; solutions aliquoted at −20 °C, avoid freeze–thaw

Typical packs

1 g, 5 g, 25 g, 100 g

Typical literature values. The batch COA / SDS governs.

Before you inquire

Pack sizes and catalogue numbers

Prices on request; lead time confirmed on the quotation.

For research or industrial use only. Not for human or animal diagnosis or treatment, and not for drug or food use. Grade, pack size and lead time follow the supplier batch COA / SDS. Figures on this site are general industry references, not a factory specification.