E. coli induction asks for IPTG. Prokaryotic selection asks for kanamycin, carbenicillin, chloramphenicol or spectinomycin salts. Stable eukaryotic lines ask for G418 or hygromycin B.
Where each item sits
- IPTG: powder. Ask about dioxane residue and sterile filtration.
- Prokaryotic selection: kanamycin, carbenicillin, chloramphenicol, spectinomycin, tetracycline
- Eukaryotic selection: G418 and hygromycin B
- Lysis can inquire lysozyme. Inclusion bodies can inquire guanidine HCl or urea.
Questions on this application
Carbenicillin instead of ampicillin?
Carbenicillin is more stable on plates and gives fewer satellite colonies.
G418 powder or solution?
Both can be inquired. State the concentration if you need a stock solution.
For research or industrial use only. Not for human or animal diagnosis or treatment, and not for drug or food use. Grade, pack size and lead time follow the supplier batch COA / SDS. Figures on this site are general industry references, not a factory specification.